A comparison of metal nanoparticle-modified boron-doped diamond microelectrodes for the electrochemical detection of hydrogen peroxide

Document Type


Publication Date


Publication Title

Pittcon 2021


Reactive oxygen and nitrogen species (ROS/RNS) are normal byproducts of oxygen metabolism in aerobic cells; however, ROS/RNS produced in large amounts can lead to oxidative stress. This is of main concern as oxidative stress is a contributor to neurodegenerative diseases such as Alzheimer’s and Parkinson’s, as well as some forms of cancer. Developing a method that can detect small quantities of ROS such as hydrogen peroxide (H2O2) would allow for the possibility of in vivo detection methods that can be performed at the cellular level. Because many ROS/RNS (including H2O2) are electroactive, electrochemical detection offers several advantages including low cost, capability to be miniaturized, and in some cases, subsecond time scale measurements. Additionally, the size of microelectrodes and the speed at which electrochemical techniques can be performed provide the spatio-temporal resolution required. Modification of the electrode surface can alter the specificity, allowing for a more specialized electrode for the analyte of interest. For H2O2 specifically, metal nanoparticles (NPs), such as silver (Ag), gold (Au), palladium (Pd), and platinum (Pt), can be deposited onto the electrode surface to increase sensitivity and selectivity. Literature exists for the use of each of these metal NPs for H2O2 detection, but a comprehensive comparison has yet to be completed in a single study. In this work, we compared the performance of boron-doped diamond (BDD) electrodes modified with Ag, Au, Pd, and Pt NPs for detection of H2O2. BDD is an excellent substrate for NP modification as it offers a smaller capacitive current, enhanced surface roughness, and a large potential window. Several factors were considered when choosing the highest performing NP-modified BDD electrodes including, sensitivity, detection limit, a penchant for fouling, and repeatability. This work expands on the bounds of electroanalytical detection and the applicability of diamond as a material for biosensing.

Controlled Subject

Nanoparticles; Microelectrodes; Hydrogen peroxide


Nanoscience and Nanotechnology

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