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Description

Wastewater surveillance for poliovirus is a useful tool for spatial surveillance and evaluation of immunization efficacy. Polio testing in wastewater has been conducted since the 1980s in Finland and Israel, and the virus was recently discovered in New York. With increasing concern regarding the vaccination of children against the virus, there has been worry that polio cases could once again present themselves in the Las Vegas Valley. By testing for the virus in wastewater, we can monitor any new cases or outbreaks that occur and inform authorities as to the location and viral count. The wastewater samples were collected at various locations throughout the Las Vegas Valley and brought to the lab for analysis. Nucleic acids from the samples were isolated and purified, and complementary DNA was for further testing. The DNA was tested using quantitative polymerase chain reaction to determine the concentration of poliovirus in the starting sample. After several months of testing, no polio was identified in the wastewater. The qPCR data indicated that no poliovirus was replicated. This data is useful moving forward because it reveals that there were no major polio outbreaks in the areas that were tested. Further research would need to be conducted in order to ensure continuing safety from poliovirus in Las Vegas.

Publisher Location

Las Vegas (Nev.)

Publication Date

12-9-2022

Publisher

University of Nevada, Las Vegas

Language

English

Controlled Subject

Viral pollution of water; Water--Testing

Disciplines

Viruses | Water Resource Management

File Format

pdf

File Size

1000 KB

Comments

Faculty Mentors: Edwin C. Oh, Van Vo

Rights

IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

Poliovirus Detection in Las Vegas Wastewater


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