PCR Monitoring of Parasitemia during Drug Treatment for Canine Chagas Disease

Authors

Chih-Ling Zao, VRL-San AntonioFollow
Ya-Chin Yang, VRL-San Antonio
Lisa Tomanek, VRL-San Antonio
Anthony Cooke, VRL-San Antonio
Ron Berger, VRL-San Antonio
Lung-Chang Chien, Unviersity of Nevada, Las VegasFollow
Roy Madigan, Animal Hospital of Smithson Valley

Document Type

Article

Publication Date

8-5-2019

Publication Title

Journal of Veterinary Diagnostic Investigation

First page number:

1

Last page number:

5

Abstract

To date, there is no clear standard to monitor drug treatment for canine Chagas disease. We used 2 real-time PCR (rtPCR) assays targeting Trypanosoma cruzi kinetoplast DNA (kDNA) and nuclear satellite DNA (nDNA) to detect T. cruzi in canine whole blood. Samples were collected randomly from 131 untreated dogs with unknown T. cruzi infection status in Texas. ... See full text for complete abstract.

Keywords

Canine Changas disease; Kinetoplast DNA; Nuclear satellite DNA; Real-time PCR; Trypanosoma cruzi

Disciplines

Veterinary Infectious Diseases

Language

English

Repository Citation

Zao, C., Yang, Y., Tomanek, L., Cooke, A., Berger, R., Chien, L., Madigan, R. (2019). PCR Monitoring of Parasitemia during Drug Treatment for Canine Chagas Disease. Journal of Veterinary Diagnostic Investigation 1-5.
http://dx.doi.org/10.1177/1040638719868508