Location
University of Nevada, Las Vegas
Start Date
16-4-2011 10:00 AM
End Date
16-4-2011 11:30 AM
Description
Oxygen delivery rates must keep pace with the increased metabolic rate observed in poikilothermic animals exposed to increased temperature if aerobic metabolism is to be maintained. Physical failure of the oxygen delivery system to meet these demands may be a mechanism of cardiac failure in crustaceans at temperature extremes. As part of the determination of the effect of temperature on the cardio-respiratory system, we are measuring whole animal lactate levels in grass shrimp acclimated to 20°C over temperature ranges in normoxic and hyperoxic conditions. Lactate levels are measured as an indicator of a switch from aerobic to anaerobic metabolism. Animals placed in a hyperoxic environment had a higher CT max than animals in a normoxic environment, suggesting a physical limitation in oxygen delivery. Animals were collected and placed in liquid nitrogen across the temperature range in both normoxic and hyperoxic conditions. Lactate levels were determined with whole animals using an enzymatic method from a commercial lactate analysis kit.
Keywords
Palaemonetes; Shrimps — Effect of high temperatures on; Water — Dissolved oxygen
Disciplines
Animal Sciences | Biochemistry | Physiology
Language
English
Effect of temperature and oxygen levels on lactate production in Palaemonetes pugio
University of Nevada, Las Vegas
Oxygen delivery rates must keep pace with the increased metabolic rate observed in poikilothermic animals exposed to increased temperature if aerobic metabolism is to be maintained. Physical failure of the oxygen delivery system to meet these demands may be a mechanism of cardiac failure in crustaceans at temperature extremes. As part of the determination of the effect of temperature on the cardio-respiratory system, we are measuring whole animal lactate levels in grass shrimp acclimated to 20°C over temperature ranges in normoxic and hyperoxic conditions. Lactate levels are measured as an indicator of a switch from aerobic to anaerobic metabolism. Animals placed in a hyperoxic environment had a higher CT max than animals in a normoxic environment, suggesting a physical limitation in oxygen delivery. Animals were collected and placed in liquid nitrogen across the temperature range in both normoxic and hyperoxic conditions. Lactate levels were determined with whole animals using an enzymatic method from a commercial lactate analysis kit.