Sars-Cov-2 Variant Detection at a University Dormitory Using Wastewater Genomic Tools
Document Type
Article
Publication Date
1-20-2022
Publication Title
Science of the Total Environment
Volume
805
Abstract
In the Fall of 2020, university campuses in the United States resumed on-campus instruction and implemented wastewater monitoring for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While quantitative polymerase chain reaction (qPCR) tests were deployed successfully to detect viral RNA in wastewater across campuses, the feasibility of detecting viral variants from a residential building like a dormitory was unclear. Here, we demonstrate that wastewater surveillance from a dormitory with at least three infected students could lead to the identification of viral genomes with more than 95% coverage. Our results indicate that viral variant detection from wastewater is achievable at a dormitory and that coronavirus disease 2019 (COVID-19) wastewater surveillance programs will benefit from the implementation of viral whole genome sequencing at universities.
Keywords
COVID-19; Mutation; SARS-CoV-2; Sewage; Variant; Virus; Wastewater
Disciplines
Viruses | Water Resource Management
Repository Citation
Vo, V.,
Tillett, R. L.,
Chang, C.,
Gerrity, D.,
Betancourt, W. Q.,
Oh, E. C.
(2022).
Sars-Cov-2 Variant Detection at a University Dormitory Using Wastewater Genomic Tools.
Science of the Total Environment, 805
Available at:
http://dx.doi.org/10.1016/j.scitotenv.2021.149930