"Kinetic analysis of lactate dehydrogenase using integrated rate equati" by Leonard D. Holmes, Martin R. Schiller et al.
 

Kinetic analysis of lactate dehydrogenase using integrated rate equations.

Document Type

Article

Publication Date

10-15-1993

Publication Title

Experientia

Volume

49

Issue

10

First page number:

893

Last page number:

901

Abstract

The reaction catalyzed by lactate dehydrogenase was analyzed under fully second-order conditions using integrated rate equations. A two-step regression analysis was utilized to fit twenty-one progress curves repeated in sextuplicate to the general mechanism second-order integrated rate equation with additional terms of substrate inhibition. The fitting error was less than one percent. The resulting kinetic constants support a ternary complex mechanism; in no case were constants supporting another mechanism predicted. The inhibition constant for oxamate was also determined.

Keywords

Animals; Binding; Catalysis; Competitive; Isoenzymes/antagonists & inhibitors; Isoenzymes/metabolism; Kinetics; L-Lactate Dehydrogenase/antagonists & inhibitors; L-Lactate Dehydrogenase/metabolism; Lactate dehydrogenase; Mathematics; NAD/metabolism; Pyruvates/metabolism; Pyruvic Acid; Rabbits; Regression Analysis; Serum albumin; Serum Albumin; Bovine/pharmacology

Disciplines

Applied Mathematics | Biochemistry | Chemistry | Life Sciences

Language

English

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