Kinetic analysis of lactate dehydrogenase using integrated rate equations.
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The reaction catalyzed by lactate dehydrogenase was analyzed under fully second-order conditions using integrated rate equations. A two-step regression analysis was utilized to fit twenty-one progress curves repeated in sextuplicate to the general mechanism second-order integrated rate equation with additional terms of substrate inhibition. The fitting error was less than one percent. The resulting kinetic constants support a ternary complex mechanism; in no case were constants supporting another mechanism predicted. The inhibition constant for oxamate was also determined.
Animals; Binding; Catalysis; Competitive; Isoenzymes/antagonists & inhibitors; Isoenzymes/metabolism; Kinetics; L-Lactate Dehydrogenase/antagonists & inhibitors; L-Lactate Dehydrogenase/metabolism; Lactate dehydrogenase; Mathematics; NAD/metabolism; Pyruvates/metabolism; Pyruvic Acid; Rabbits; Regression Analysis; Serum albumin; Serum Albumin; Bovine/pharmacology
Applied Mathematics | Biochemistry | Chemistry | Life Sciences
Holmes, L. D.,
Schiller, M. R.,
Boeker, E. A.
Kinetic analysis of lactate dehydrogenase using integrated rate equations..