Measuring spectroscopy and magnetism of extracted and intracellular magnetosomes using soft X-ray ptychography

Authors

X Zhu, McMaster UniversityFollow
A P. Hitchcock, McMaster UniversityFollow
Dennis A Bazylinski, University of Nevada, at Las VegasFollow
P Denes, Lawrence Berkeley National Laboratory
J Joseph, Lawrence Berkeley National Laboratory
U Lins, Universidade Federal do Rio de JaneiroFollow
S Marchesini, Lawrence Berkeley National LaboratoryFollow
H W. Shiu, Lawrence Berkeley National Laboratory
T Tyliszczak, Lawrence Berkeley National LaboratoryFollow
D A. Shapiro, Lawrence Berkeley National Laboratory

Document Type

Article

Publication Date

1-1-2016

Publication Title

Proceedings of the National Academy of Sciences of the United States of America

Volume

113

Issue

51

First page number:

E8219

Last page number:

E8227

Abstract

Characterizing the chemistry and magnetism of magnetotactic bacteria (MTB) is an important aspect of understanding the biomineralization mechanism and function of the chains of magnetosomes (Fe3O4 nanoparticles) found in such species. Images and X-ray absorption spectra (XAS) of magnetosomes extracted from, and magnetosomes in, whole Magnetovibrio blakemorei strain MV-1 cells have been recorded using soft X-ray ptychography at the Fe 2p edge. A spatial resolution of 7 nm is demonstrated. Precursor-like and immature magnetosome phases in a whole MV-1 cell were visualized, and their Fe 2p spectra were measured. Based on these results, a model for the pathway of magnetosome biomineralization for MV-1 is proposed. Fe 2p X-ray magnetic circular dichroism (XMCD) spectra have been derived from ptychography image sequences recorded using left and right circular polarization. The shape of the XAS and XMCD signals in the ptychographic absorption spectra of both sample types is identical to the shape and signals measured with conventional bright-field scanning transmission X-ray microscope. A weaker and inverted XMCD signal was observed in the ptychographic phase spectra of the extracted magnetosomes. The XMCD ptychographic phase spectrum of the intracellular magnetosomes differed from the ptychographic phase spectrum of the extracted magnetosomes. These results demonstrate that spectro-ptychography offers a superior means of characterizing the chemical and magnetic properties of MTB at the individual magnetosome level. © 2016, National Academy of Sciences. All rights reserved.

Keywords

Biomineralization; Magnetotactic bacteria; Ptychography; STXM; XAS-XMCD

Language

English

Repository Citation

Zhu, X., Hitchcock, A. P., Bazylinski, D., Denes, P., Joseph, J., Lins, U., Marchesini, S., Shiu, H. W., Tyliszczak, T., Shapiro, D. A. (2016). Measuring spectroscopy and magnetism of extracted and intracellular magnetosomes using soft X-ray ptychography. Proceedings of the National Academy of Sciences of the United States of America, 113(51), E8219-E8227.
http://dx.doi.org/10.1073/pnas.1610260114