Role of Base Excision Repair (BER) in Transcription-associated Mutagenesis of Nutritionally Stressed Nongrowing Bacillus subtilis Cell Subpopulations

Authors

V Ambriz-Aviña, University of Guanajuato
R E. Yasbin, University of Missouri-St LouisFollow
Eduardo Robleto, University of Nevada, Las VegasFollow
M Pedraza-Reyes, University of GuanajuatoFollow

Document Type

Article

Publication Date

1-1-2016

Publication Title

Current Microbiology

Volume

73

Issue

5

First page number:

721

Last page number:

726

Abstract

Compelling evidence points to transcriptional processes as important factors contributing to stationary-phase associated mutagenesis. However, it has not been documented whether or not base excision repair mechanisms play a role in modulating mutagenesis under conditions of transcriptional derepression. Here, we report on a flow cytometry-based methodology that employs a fluorescent reporter system to measure at single-cell level, the occurrence of transcription-associated mutations in nutritionally stressed B. subtilis cultures. Using this approach, we demonstrate that (i) high levels of transcription correlates with augmented mutation frequency, and (ii) mutation frequency is enhanced in nongrowing population cells deficient for deaminated (Ung, YwqL) and oxidized guanine (GO) excision repair, strongly suggesting that accumulation of spontaneous DNA lesions enhance transcription-associated mutagenesis. © 2016, Springer Science+Business Media New York.

Language

English

Repository Citation

Ambriz-Aviña, V., Yasbin, R. E., Robleto, E., Pedraza-Reyes, M. (2016). Role of Base Excision Repair (BER) in Transcription-associated Mutagenesis of Nutritionally Stressed Nongrowing Bacillus subtilis Cell Subpopulations. Current Microbiology, 73(5), 721-726.
http://dx.doi.org/10.1007/s00284-016-1122-9