Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Authors

R M. Bowers, Department of Energy Joint Genome Institute
N C. Kyrpides, Department of Energy Joint Genome InstituteFollow
R Stepanauskas, Bigelow Laboratory for Ocean Sciences
M Harmon Smith, Department of Energy Joint Genome Institute
D Doud, Department of Energy Joint Genome Institute
T B.K. Reddy, Department of Energy Joint Genome Institute
F Schulz, Department of Energy Joint Genome Institute
J Jarett, Department of Energy Joint Genome InstituteFollow
A R. Rivers, Department of Energy Joint Genome Institute
E A. Eloe Fanrosh, Department of Energy Joint Genome InstituteFollow
S G. Tringe, University of California, Merced
N N. Ivanova, Department of Energy Joint Genome InstituteFollow
A Copeland, Department of Energy Joint Genome Institute
A Clum, Department of Energy Joint Genome InstituteFollow
E D. Becraft, Bigelow Laboratory for Ocean SciencesFollow
R R. Malmstrom, Department of Energy Joint Genome Institute
B Birren, Broad Institute
M Podar, Oak Ridge National Laboratory
P Bork, European Molecular Biology Laboratory
G M. Weinstock, Jackson Laboratory for Genomic Medicine
G M. Garrity, Michigan State University
J A. Dodsworth, California State University, San BernardinoFollow
S Yooseph, Craig Venter Institute
G Sutton, Craig Venter Institute
F O. Glockner, Institute for Marine Microbiology
J A. Gilbert, University of Chicago
W C. Nelson, Pacific Northwest National Laboratory
S J. Hallam, University of British ColumbiaFollow
S P. Jungbluth, University of Southern California
T J.G. Ettema, Uppsala UniversityFollow
S Tighe, University of Vermont
Konstantinidis

Document Type

Article

Publication Date

1-1-2017

Publication Title

Nature Biotechnology

Publisher

Nature Publishing Group

Volume

35

Issue

8

First page number:

725

Last page number:

731

Abstract

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Language

english

Repository Citation

Bowers, R. M., Kyrpides, N. C., Stepanauskas, R., Harmon Smith, M., Doud, D., Reddy, T. B., Schulz, F., Jarett, J., Rivers, A. R., Eloe Fanrosh, E. A., Tringe, S. G., Ivanova, N. N., Copeland, A., Clum, A., Becraft, E. D., Malmstrom, R. R., Birren, B., Podar, M., Bork, P., Weinstock, G. M., Garrity, G. M., Dodsworth, J. A., Yooseph, S., Sutton, G., Glockner, F. O., Gilbert, J. A., Nelson, W. C., Hallam, S. J., Jungbluth, S. P., Ettema, T. J., Tighe, S., Konstantinidis (2017). Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea. Nature Biotechnology, 35(8), 725-731. Nature Publishing Group.
http://dx.doi.org/10.1038/nbt.3893