Reversible reduction of estrone to 17β-estradiol by Rhizobium, Sphingopyxis, and Pseudomonas isolates from the Las Vegas Wash

Authors

S M. Blunt, University of Nevada, Las VegasFollow
M J. Benotti, NewFields Environmental Forensics PracticeFollow
M R. Rosen, Southern Nevada Water AuthorityFollow
B P. Hedlund, University of Nevada, Las VegasFollow
D P. Moser, University of Nevada, Las VegasFollow

Document Type

Article

Publication Date

1-1-2017

Publication Title

Journal of Environmental Quality

Volume

46

Issue

2

First page number:

281

Last page number:

287

Abstract

Environmental endocrine-disrupting compounds (EDCs) are a growing concern as studies reveal their persistence and detrimental effects on wildlife. Microorganisms are known to affect the transformation of steroid EDCs; however, the diversity of estrogen-degrading microorganisms and the range of transformations they mediate remain relatively little studied. In mesocosms, low concentrations of PC minimally reduced E1 to E2 but effectively oxidized E2 to E1 after a 20-d lag. In the absence of alternative carbon sources, LVW-12 and LVW-PC oxidized E2 to E1. This report documents the rapid and sometimes reversible microbial tradded estrone (E1) and 17β-estradiol (E2) were removed by indigenous microorganisms from Las Vegas Wash water within 2 wk. Three bacterial isolates, Rhizobium sp. strain LVW-9, Sphingopyxis sp. strain LVW-12, and Pseudomonas sp. strain LVW-PC, were enriched from Las Vegas Wash water on E1 and E2 and used for EDC transformation studies. In the presence of alternative carbon sources, LVW-9 and LVW-12 catalyzed near-stoichiometric reduction of E1 to E2 but subsequently reoxif steroid estrogens via wastewater could be reduced through tighter control of redox conditions and may assist in future risk assessments detailing the environmental fate of estrogens through evidence that microbial estrogen transformations may be affected bydized E2 back to E1; whereas LVWansformation of E1 and E2 and the slow degradation of 17α-ethinylestradiol in urban stream water and extends the list of known estrogentransforming bacteria to the genera Rhizobium and Sphingopyxis. These results suggest that discharge o environmental conditions or growth status. © American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. 5585 Guilford Rd., Madison, WI 53711 USA. All rights reserved.

Language

english

Repository Citation

Blunt, S. M., Benotti, M. J., Rosen, M. R., Hedlund, B. P., Moser, D. P. (2017). Reversible reduction of estrone to 17β-estradiol by Rhizobium, Sphingopyxis, and Pseudomonas isolates from the Las Vegas Wash. Journal of Environmental Quality, 46(2), 281-287.
http://dx.doi.org/10.2134/jeq2016.08.0286