Document Type
Article
Publication Date
9-24-2018
Publication Title
Antimicrobial Agents and Chemotherapy
Volume
62
Issue
10
First page number:
1
Last page number:
12
Abstract
Clostridium difficile infection (CDI) is the leading cause of antibiotic-associated diarrhea and has gained worldwide notoriety due to emerging hypervirulent strains and the high incidence of recurrence. We previously reported protection of mice from CDI using the antigerminant bile salt analog CamSA. Here we describe the effects of CamSA in the hamster model of CDI. CamSA treatment of hamsters showed no toxicity and did not affect the richness or diversity of gut microbiota; however, minor changes in community composition were observed. Treatment of C. difficile-challenged hamsters with CamSA doubled the mean time to death, compared to control hamsters. However, CamSA alone was insufficient to prevent CDI in hamsters. CamSA in conjunction with suboptimal concentrations of vancomycin led to complete protection from CDI in 70% of animals. Protected animals remained disease-free at least 30 days postchallenge and showed no signs of colonic tissue damage. In a delayed-treatment model of hamster CDI, CamSA was unable to prevent infection signs and death. These data support a putative model in which CamSA reduces the number of germinating C. difficile spores but does not keep all of the spores from germinating. Vancomycin halts division of any vegetative cells that are able to grow from spores that escape CamSA.
Keywords
C. difficile; Microbiome; Antigermination; CamSA; Hamster
Disciplines
Life Sciences | Medical Pharmacology
File Format
File Size
2.059 Kb
Language
English
Permissions
Copyright © 2018 American Society for Microbiology.
Publisher Citation
Howerton A, Seymour CO, Murugapiran SK, Liao Z, Phan JR, Estrada A, Wagner AJ, Mefferd CC, Hedlund BP, Abel-Santos E. 2018. Effect of the synthetic bile salt analog CamSA on the hamster model of Clostridium difficile infection. Antimicrob Agents Chemother 62:e02251-17. https://doi .org/10.1128/AAC.02251-17.
Repository Citation
Howerton, A.,
Seymour, C. O.,
Murugapiran, S. K.,
Liao, Z.,
Phan, J. R.,
Estrada, A.,
Wagner, A. J.,
Mefferd, C. C.,
Hedlund, B. P.,
Abel-Santos, E.
(2018).
Effect of the Synthetic Bile Salt Analog CamSA on the Hamster Model of Clostridium difficile Infection.
Antimicrobial Agents and Chemotherapy, 62(10),
1-12.
http://dx.doi.org/10.1128/AAC.02251-17