Document Type

Article

Publication Date

9-24-2018

Publication Title

Antimicrobial Agents and Chemotherapy

Volume

62

Issue

10

First page number:

1

Last page number:

12

Abstract

Clostridium difficile infection (CDI) is the leading cause of antibiotic-associated diarrhea and has gained worldwide notoriety due to emerging hypervirulent strains and the high incidence of recurrence. We previously reported protection of mice from CDI using the antigerminant bile salt analog CamSA. Here we describe the effects of CamSA in the hamster model of CDI. CamSA treatment of hamsters showed no toxicity and did not affect the richness or diversity of gut microbiota; however, minor changes in community composition were observed. Treatment of C. difficile-challenged hamsters with CamSA doubled the mean time to death, compared to control hamsters. However, CamSA alone was insufficient to prevent CDI in hamsters. CamSA in conjunction with suboptimal concentrations of vancomycin led to complete protection from CDI in 70% of animals. Protected animals remained disease-free at least 30 days postchallenge and showed no signs of colonic tissue damage. In a delayed-treatment model of hamster CDI, CamSA was unable to prevent infection signs and death. These data support a putative model in which CamSA reduces the number of germinating C. difficile spores but does not keep all of the spores from germinating. Vancomycin halts division of any vegetative cells that are able to grow from spores that escape CamSA.

Keywords

C. difficile; Microbiome; Antigermination; CamSA; Hamster

Disciplines

Life Sciences | Medical Pharmacology

File Format

pdf

File Size

2.059 Kb

Language

English

Rights

Copyright © 2018 American Society for Microbiology.

Publisher Citation

Howerton A, Seymour CO, Murugapiran SK, Liao Z, Phan JR, Estrada A, Wagner AJ, Mefferd CC, Hedlund BP, Abel-Santos E. 2018. Effect of the synthetic bile salt analog CamSA on the hamster model of Clostridium difficile infection. Antimicrob Agents Chemother 62:e02251-17. https://doi .org/10.1128/AAC.02251-17.

UNLV article access

Search your library

Share

COinS