Document Type
Article
Publication Date
11-4-2024
Publication Title
Nature Communications
Volume
15
First page number:
1
Last page number:
17
Abstract
Few aerobic hyperthermophilic microorganisms degrade polysaccharides. Here, we describe the genome-enabled enrichment and optical tweezer-based isolation of an aerobic polysaccharide-degrading hyperthermophile, Fervidibacter sacchari, previously ascribed to candidate phylum Fervidibacteria. F. sacchari uses polysaccharides and monosaccharides for growth at 65–87.5 °C and expresses 191 carbohydrate-active enzymes (CAZymes) according to RNA-Seq and proteomics, including 31 with unusual glycoside hydrolase domains (GH109, GH177, GH179). Fluorescence in-situ hybridization and nanoscale secondary ion mass spectrometry confirmed rapid assimilation of 13C-starch in spring sediments. Purified GHs were optimally active at 80–100 °C on ten different polysaccharides. Finally, we propose reassigning Fervidibacteria as a class within phylum Armatimonadota, along with 18 other species, and show that a high number and diversity of CAZymes is a hallmark of the phylum, in both aerobic and anaerobic lineages. Our study establishes Fervidibacteria as hyperthermophilic polysaccharide degraders in terrestrial geothermal springs and suggests a broad role for Armatimonadota in polysaccharide catabolism.
Controlled Subject
Microorganisms; Polysaccharides--Structure; Microbial biotechnology
Disciplines
Microbial Physiology | Molecular Biology
File Format
File Size
2900 KB
Language
English
Rights
IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Repository Citation
Nou, N. O.,
Covington, J. K.,
Lai, D.,
Mayali, X.,
Seymour, C. O.,
Johnston, J.,
Jiao, J.,
Buessecker, S.,
Mosier, D.,
Muok, A. R.,
Torosian, N.,
Cook, A. M.,
Briegel, A.,
Woyke, T.,
Eloe-Fadrosh, E.,
Shapiro, N.,
Bryan, S. G.,
Sleezer, S.,
Dimapilis, J.,
Gonzalez, C.,
Gonzalez, L.,
Noriega, M.,
Hess, M.,
Carlson, R. P.,
Liu, L.,
Li, M.,
Lian, Z.,
Zhu, S.,
Liu, F.,
Sun, X.,
Gao, B.,
Mewalal, R.,
Harmon-Smith, M.,
Blaby, I. K.,
Cheng, J.,
Weber, P. K.,
Grigorean, G.,
Li, W.,
Dekas, A. E.,
Pett-Ridge, J. E.,
Dodsworth, J. A.,
Palmer, M.,
Hedlund, B. P.
(2024).
Genome-Guided Isolation of the Hyperthermophilic Aerobe Fervidibacter sacchari Reveals Conserved Polysaccharide Metabolism in the Armatimonadota.
Nature Communications, 15
1-17.
http://dx.doi.org/10.1038/s41467-024-53784-3
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