"Data Supporting a Saturation Mutagenesis Assay for Tat-Driven Transcri" by Ronald Benjamin, Christopher J. Giacoletto et al.
 

Document Type

Article

Publication Date

9-28-2022

Publication Title

Data in Brief

Volume

45

First page number:

1

Last page number:

25

Abstract

The data in this article are associated with the research paper “GigaAssay – an adaptable high-throughput saturation mutagenesis assay". The raw data are sequence reads of HIV-1 Tat cDNA amplified from cellular genomic DNA in a new single-pot saturation mutagenesis assay designated the “GigaAssay”. A bioinformatic pipeline and parameters used to analyze the data. Raw, processed, analyzed, and filtered data are reported. The data is processed to calculate the Tat-driven transcription activity for cells with each possible single amino acid substitution in Tat. This data can be reused to interpret Tat intermolecular interactions and HIV latency. This is one of the largest and most complete datasets regarding the impact of amino acid substitutions within a single protein on a molecular function.

Keywords

Tat Transcription; High-throughput assay; Loss of Function (LOF); Saturation mutagenesis; Protein structure; Intragenic epistasis

Disciplines

Cell Anatomy | Cell Biology | Molecular Biology | Other Biochemistry, Biophysics, and Structural Biology

File Format

PDF

File Size

7980 KB

Language

English

Rights

IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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