Document Type
Article
Publication Date
9-28-2022
Publication Title
Data in Brief
Volume
45
First page number:
1
Last page number:
25
Abstract
The data in this article are associated with the research paper “GigaAssay – an adaptable high-throughput saturation mutagenesis assay". The raw data are sequence reads of HIV-1 Tat cDNA amplified from cellular genomic DNA in a new single-pot saturation mutagenesis assay designated the “GigaAssay”. A bioinformatic pipeline and parameters used to analyze the data. Raw, processed, analyzed, and filtered data are reported. The data is processed to calculate the Tat-driven transcription activity for cells with each possible single amino acid substitution in Tat. This data can be reused to interpret Tat intermolecular interactions and HIV latency. This is one of the largest and most complete datasets regarding the impact of amino acid substitutions within a single protein on a molecular function.
Keywords
Tat Transcription; High-throughput assay; Loss of Function (LOF); Saturation mutagenesis; Protein structure; Intragenic epistasis
Disciplines
Cell Anatomy | Cell Biology | Molecular Biology | Other Biochemistry, Biophysics, and Structural Biology
File Format
File Size
7980 KB
Language
English
Rights
IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Repository Citation
Benjamin, R.,
Giacoletto, C. J.,
FitzHugh, Z. T.,
Eames, D.,
Buczek, L.,
Wu, X.,
Newsome, J.,
Han, M. V.,
Pearson, T.,
Wei, Z.,
Banerjee, A.,
Brown, L.,
Valente, L. J.,
Shen, S.,
Deng, H.,
Schiller, M. R.
(2022).
Data Supporting a Saturation Mutagenesis Assay for Tat-Driven Transcription With the GigaAssay.
Data in Brief, 45
1-25.
http://dx.doi.org/10.1016/j.dib.2022.108641
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