Document Type
Article
Publication Date
9-3-2019
Publication Title
Scientific Reports
Publisher
Nature Research
Volume
9
First page number:
1
Last page number:
9
Abstract
Recent studies imply that rare variants contribute to the risk of schizophrenia, however, the exact variants or genes responsible for this condition are largely unknown. In this study, we conducted whole genome sequencing (WGS) of 20 Chinese families. Each family consisted of at least two affected siblings diagnosed with schizophrenia and at least one unaffected sibling. We examined functional variants that were found in affected sibling(s) but not in unaffected sibling(s) within a family. Matching this criterion, a frameshift heterozygous deletion of CA (–/CA) at chromosome 18:24722722, also referred to as rs752084147, in the Carbohydrate Sulfotransferase 9 (CHST9) gene, was detected in two families. This deletion was confirmed by PCR-based Sanger sequencing. With the observed frequency of 0.00076 in Han Chinese population, we performed both case-control and family-based analyses to evaluate its association with schizophrenia. In the case-control analyses, Chi-square test P-value was 6.80e-12 and the P-value was 0.0008 after one million simulations. In family-based segregation analyses, segregation P-value was 7.72e-7 and simulated P-value was 5.70e-6. For both the case-control and family-based analyses, the CA deletion was significantly associated with schizophrenia in the Chinese population. Further investigation of this gene is warranted in the development of schizophrenia by utilizing larger and more ethnically diverse samples.
Disciplines
Genomics
File Format
File Size
1.203 KB
Language
English
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Repository Citation
Chen, J.,
Wu, J.,
Mize, T.,
Moreno, M.,
Hamid, M.,
Servin, F.,
Bashy, B.,
Zhao, Z.,
Jia, P.,
Tsuang, M. T.,
Kendler, K. S.,
Xiong, M.,
Chen, X.
(2019).
A Frameshift Variant in the CHST9 Gene Identified by Family-Based Whole Genome Sequencing Is Associated with Schizophrenia in Chinese Population.
Scientific Reports, 9
1-9.
Nature Research.
http://dx.doi.org/10.1038/s41598-019-49052-w