17β-Estradiol Alters Mrna Co-Expression After Murine Muscle Injury and Mild Hypobaria
Document Type
Article
Publication Date
9-18-2019
Publication Title
Experimental Biology and Medicine
Abstract
Here, we assessed the effects of 17β-estradiol exposure on mRNA co-expression patterns of muscle tissue during recovery in a closed muscle crush injury and hypobaria exposure murine model. Eighteen ovariectomized placebo-treated and 18 ovariectomized 17β-estradiol-treated female mice underwent closed muscle crush injury and hypobaric simulated flight. The mice recovered for 32, 96, or 192 h, and then were euthanized. Their harvested injured lateral gastrocnemius muscles underwent microarray analysis. We used weighted gene co-expression network analysis to construct a co-expression network for the control mice, and then applied the same network to the estrogen-treated mice. We compared the relationships between co-expression in gene modules over time between the two experimental groups. Enriched functional cluster analyses of significant co-expression network modules document a variety of different pathways of interest. Some of the functional cluster enrichments within several of the significantly correlated modules are related to the formation and function of microtubules. Our findings demonstrate that following a closed muscle crush injury in a murine model, the presence of 17β-estradiol alters mRNA co-expression patterns over time. It appears that estrogen promotes the expression of mRNA related to microtubule activity within the cytoskeleton of myofibers and in movement of organelles and receptors. Further study is needed, but the enrichment of these microtubule-related pathways may be integral in the muscle tissue regeneration process, and thus suggests that the presence of estrogen may promote muscle recovery through the work of the microtubules.
Keywords
Closed muscle crush injury; Estrogen; Hypobaria; Microtubules
Disciplines
Medical Sciences | Medicine and Health Sciences | Musculoskeletal, Neural, and Ocular Physiology
Language
English
Repository Citation
Moore, S. E.,
Voss, J. G.,
St. Pierre Schneider, B.
(2019).
17β-Estradiol Alters Mrna Co-Expression After Murine Muscle Injury and Mild Hypobaria.
Experimental Biology and Medicine
http://dx.doi.org/10.1177/1535370219877360