Master of Science (MS)
First Committee Member
Second Committee Member
Third Committee Member
Fourth Committee Member
Fifth Committee Member
Sixth Committee Member
Kathryn H. Korgan
Number of Pages
Mesenchymal stem cells are derived from a variety of human tissues and are being bioengineered and studied for possible uses in the advancement of medicine. Recent efforts are being focused on Dental Pulp Stem Cells (DPSC's) due to the accessibility of this tissue. Many factors inﬂuence DPSC quality and quantity, including the speciﬁc methods used to isolate, collect, concentrate, and store these isolates once they are removed. Ancillary factors, such as the choice of media, the selection of early versus late passage cells, and cryopreservation techniques may also influence the differentiation potential and proliferative capacity of DPSC isolates.
The objective of this study was to evaluate the potential to induce differentiation of DPSC isolates in vitro by the adding of exogenous growth factors (GF), and by the coating of specific extracellular matrix molecules (ECM) onto the surface of tissue-culture dishes. Photomicroscopy and mRNA analysis demonstrated the addition of TGF-β1 notably increased pluripotency biomarkers in DPSC lines. The addition of Dexamethasone (Dex) or plating on Laminin-5 (LN5) was correlated with changes to cellular morphology and cell size in different subsets of cells. RNA isolated from these DPSCs for relative endpoint (RE) reverse transcription polymerase chain reaction (RT-PCR) revealed mRNA DPSC specific intracellular biomarkers (Klf4, Sox-2, Bin-1, Rnf12, Oct-4 and NANOG) and the cell surface marker (CD133) were enhanced following the administration of TGF- β 1 and were differentially down-regulated following Dexamethasone and Laminin-5 administration. This study provides some initial evidence that randomly selected DPSC isolates may be induced by established protocols to change phenotype and expression of pluripotent biomarkers with variable susceptibility between differing types of DPSCs. More studies will be needed to determine the range of cell types that can be successfully re-engineered in laboratory settings.
Cell differentiation; Dental pulp; Dental pulp Stem Cells; Differentiation; Extracellular matrix; Growth factors; Mesenchymal stem cells; Stem cells
Biomechanical Engineering | Biomedical | Biomedical Devices and Instrumentation | Cell and Developmental Biology | Cells | Dentistry
Young, Aubrey, "Induction of Differentiation of Dental Pulp-Derived Mesenchymal Stem cells (DPSC)" (2014). UNLV Theses, Dissertations, Professional Papers, and Capstones. 2316.