Master of Science (MS)
First Committee Member
Eduardo A. Robleto
Second Committee Member
Brian P. Hedlund
Third Committee Member
Helen J. Wing
Fourth Committee Member
Number of Pages
Since the 1950’s it has been shown that bacterial cells accumulate mutations even in non- dividing conditions, but how this type of mutation occurs is still highly debated. In Bacillus subtilis, Mfd, a precursor of the nucleotide excision repair (NER) system, mediates the formation of mutations in stationary-phase or non-replicating cells. In growing cells, Mfd recruits repair when RNA polymerase is stalled during transcription; it then recruits proteins from NER to repair damage. Here, we examine the hypothesis that Mfd mediates the formation of mutations by interacting with cellular components that repair reactive oxygen species (ROS), a natural byproduct of bacteria cell respiration. Utilizing two oxidants, we tested the hypothesis that Mfd protects cell viability and mutation development after exposure to ROS in stationary-phase. Our data showed that Mfd mitigated damage caused by reactive oxygen species and that such effect is independent of the NER system. Also, Mfd, MutY and reactive oxygen species mediated the formation of mutations in stationary-phase cells.
Bacillus subtilis; Mfd; Oxidative damage repair; Stationary-phase mutagenesis; Transcription
Biology | Genetics | Microbiology
University of Nevada, Las Vegas
Porter, Katelyn E., "The Role of Mfd in Stationary-Phase Oxidative Damage Repair in Bacillus subtilis" (2016). UNLV Theses, Dissertations, Professional Papers, and Capstones. 2802.
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