Award Date

Fall 1997

Degree Type


Degree Name

Bachelor of Arts


Environmental Science

Advisor 1

Dr. William Brumley, Research Chemist, U.S. Environmental Protection Agency

Number of Pages



This thesis involves work that was accomplished during a two-year internship at the Environmental Protection Agency's (EPA), Environmental Chemistry Branch. The research is an application of trace level determination of fluorescein dyes used as groundwater tracers. The work was performed to determine whether groundwater could migrate from a Resource Conservation Recovery Act (RCRA) site to an adjacent Superfund site. The research involved using Spectrofluorimetry and a new technique called capillary electrophoresis/laser induced fluorescence (CE/LIF) to determine tracer dyes that were injected at the RCRA site and monitored at the Superfund site. Results from Spectrofluorimetry were compared to those of CE/LIF. CE provides required specificity because it is a high resolution separation technique that depends on ion mobility under free zone electrophoresis. LIF provides a sensitive detection technique for the capillary format of the separation. This study revealed fluorescein and tinopal at low parts per trillion (ppt) levels in the extracts taken from detector pads placed in the monitoring wells.


Chattanooga (Tenn.); Fluorescein; Groundwater flow; Groundwater monitoring; Groundwater pollution; Groundwater tracers methodology; Tennessee


Environmental Health and Protection | Environmental Indicators and Impact Assessment | Environmental Monitoring | Environmental Sciences | Hydrology

File Format


Degree Grantor

University of Nevada, Las Vegas




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