Award Date

1-1-1999

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

History

First Committee Member

Stephen Carper

Number of Pages

224

Abstract

We attempted to clone closely-related members of the human heat shock protein 27 (hsp 27) multigene family. Genomic DNA was obtained from human A549 lung cancer cells. Colony hybridization and the Polymerase chain reaction identified the hsp 27 genes. Positive clones were subcloned into pGEM-3Z. The DNA sequence information for three of the clones was determined, and analyzed using DNAsis and Blast Algorithms. DNA Alignments of the inserts contained in three positive clones showed a homology of 29-51% to the human hsp 27 gene. Our data suggests that these inserts are distant members of the human hsp 27 multigene family. Blast searches did not determine the identity of these inserts in GenBank and SwissProt databases (11/1/98). This result indicated that the inserts are novel sequences. Hence, the nucleotide information for the inserts was submitted to GenBank (Accession Numbers: AF120329, AF120330, AF120331, AF120332, and AF120333).

Keywords

Cloning; Family; Heat; Human; Multigene; Protein; Seven; Shock; Towards; Twenty

Controlled Subject

Biochemistry; Cellular biology; Molecular biology

File Format

pdf

File Size

5908.48 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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