Award Date

1-1-2000

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Science

First Committee Member

Steven deBelle

Number of Pages

63

Abstract

Among the retroposons, the source of the endonuclease activity is known to be variable. We investigated whether any retroposon genomes encode potential multiple endonuclease functions. Amino acid alignments were generated to reveal the presence of the characterized ordered-series-of-motifs (OSM) that define four different endonuclease functions. These endonuclease functions include a retroviral-like integrase (IN), an apurinic-apyrimidinic endonuclease (APE), the PDD endonuclease recently identified in the R2BM retroposon and an HNH endonuclease signature conserved by the group 11 intron sequences. The results indicate that the spliced l eader associated conserved sequences (SLACS) of T. brucei, c ruzi-associated retrotransposon (CZAR) of Trypanosoma cruzi, two crithidia retrotransposable elements (CRE); CRE1 and CRE2 of Crithidia fasciculata , and certain ING retroposon sequences of Trypanosoma brucei contain multiple putative endonuclease functions.

Keywords

Analysis; Bioinformatic; Containing; Endonucleases; Multiple; Putative; Retroposon; Sequences

Controlled Subject

Molecular biology; Computer science

File Format

pdf

File Size

2160.64 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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