Award Date

1-1-2001

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Health Physics and Diagnostic Sciences

First Committee Member

Steen J. Madsen

Number of Pages

57

Abstract

Rowett nude rats were injected intra-cranially with varying concentrations of 5-aminolevulinic acid (10, 20, 99, 199, 398 mM) and its hexylester (6.6, 13, 66, 132, 265 mM). Toxicity effects of these compounds were examined based on animal behavior and respiratory distress. It was determined that the maximum tolerable dose for intra-cranial injection is 99 mM 5-Aminolevulinic acid and 66 mM h-ALA; Human glioma spheroids were grown in vitro. After incubation, spheroids were sized (250--350 mum) and injected into the rat brain (10 spheroids per injection). Following the induction and development of glioblastoma multiforme tumors in vivo, Rowett nude rats were injected with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed four hours post-injection and intact brains were removed. Fluorescence microscopy was performed to quantify PpIX production. Results indicate that PpIX production in tumor tissue is greater following 5-aminolevulinic acid administration than h-ALA. However, the tumor-to-normal tissue uptake ratio is superior following injection of h-ALA (7.6 +/- 2.0:1) compared to 5-aminolevulinic acid (2.4 +/- 1.1:1).

Keywords

Acid; Administration; Aminolevulinic; Brain; Distribution; Following; Hexylester Protoporphyrin; Rat

Controlled Subject

Pharmacology; Oncology; Biophysics

File Format

pdf

File Size

1597.44 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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