Master of Science (MS)
Health Physics and Diagnostic Sciences
First Committee Member
Steen J. Madsen
Number of Pages
Rowett nude rats were injected intra-cranially with varying concentrations of 5-aminolevulinic acid (10, 20, 99, 199, 398 mM) and its hexylester (6.6, 13, 66, 132, 265 mM). Toxicity effects of these compounds were examined based on animal behavior and respiratory distress. It was determined that the maximum tolerable dose for intra-cranial injection is 99 mM 5-Aminolevulinic acid and 66 mM h-ALA; Human glioma spheroids were grown in vitro. After incubation, spheroids were sized (250--350 mum) and injected into the rat brain (10 spheroids per injection). Following the induction and development of glioblastoma multiforme tumors in vivo, Rowett nude rats were injected with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed four hours post-injection and intact brains were removed. Fluorescence microscopy was performed to quantify PpIX production. Results indicate that PpIX production in tumor tissue is greater following 5-aminolevulinic acid administration than h-ALA. However, the tumor-to-normal tissue uptake ratio is superior following injection of h-ALA (7.6 +/- 2.0:1) compared to 5-aminolevulinic acid (2.4 +/- 1.1:1).
Acid; Administration; Aminolevulinic; Brain; Distribution; Following; Hexylester Protoporphyrin; Rat
Pharmacology; Oncology; Biophysics
University of Nevada, Las Vegas
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Friesen, Scott Alan, "Protoporphyrin IX distribution in rat brain following the administration of 5-aminolevulinic acid and its hexylester" (2001). UNLV Retrospective Theses & Dissertations. 1360.