Award Date

1-1-2002

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

First Committee Member

Bryan L. Spangelo

Number of Pages

61

Abstract

The cytokines interleukin-1 (IL-1) and interleukin-6 (IL-6) are produced during an immune reaction and cause inflammation and swelling of the surrounding tissue. AD is a neurodegenerative disorder characterized by well-defined neuropathologic entities. Both IL-1 and IL-6 are present in senile plaques and may cause a self-propagating inflammatory process that contributes to the disease processes of AD; Somatostatin (SRIF) is a small, inhibitory neuropeptide present throughout the CNS. Because SRIF is an inhibitory neuropeptide we sought to determine the effects of SRIF on the production of IL-6 in rat C6 glial tumor cells. The release of IL-6 in the C6 cell conditioned media was quantified using the IL-6 dependent 7TD1 hybridoma bioassay; We decided to investigate GABA ability to inhibit the release of IL-6 by IL-1beta; To better understand the signal pathways of IL-1beta that results in the release of IL-6 we used the inhibitors SP600125 and SB203580. SB203580 is a p38 MAPK inhibitor that targets the p38alpha and p38beta2 kinases, while the p38beta kinase exhibits only partially sensitivity. The inhibitor SP600125 targets the JNK kinase. (Abstract shortened by UMI.).

Keywords

Cell; Cytokine; Disorders; Glial Indications; Neurodegenerative; Neurotransmitter; Production; Regulation

Controlled Subject

Biochemistry; Neurosciences; Cellular biology

File Format

pdf

File Size

3655.68 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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