Award Date

1-1-2004

Degree Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Civil and Environmental Engineering

First Committee Member

Jacimaria Ramos Batista

Number of Pages

254

Abstract

The impacts of different carbon sources, particularly VFAs, on EBPR's microbial population were investigated using T-RFLP and FISH. System performance and both general and specific microbial changes were investigated in SBR systems fed with acetate, propionate, butyrate, valerate, and glucose. Acetate and a mixture of acetate and propionate promoted the best P-removal. Butyrate and valerate lead the systems to fail in the long run. Glucose and propionate promoted poor EBPR, but the performance of the system fed with propionate improved with time. T-RFLP results indicated that short carbon chain VFAs, long carbon chain VFAs, and non-VFA favored different microbial populations in the systems. FISH results revealed that acetate and propionate successfully selected for the PAO, Candidatus accumulibacter phosphatis, while the other substrates tested did not. FISH analysis also showed that the GAO, Candidatus competibacter phosphatis, could not survive under any of the carbon feedings investigated, except under a feed containing a mixture of acetate and propionate.

Keywords

Acids; Bacterial; Biological; Different; Enhanced; Fatty; Impact; Phosphorus; Phosphorus Removal; Selection; Systems; Types; Volatile; Volatile Fatty Acids

Controlled Subject

Environmental engineering

File Format

pdf

File Size

8448 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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