Award Date

1-1-1997

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Science

First Committee Member

R. Keith Dupre

Second Committee Member

Penny S. Amy

Number of Pages

46

Abstract

In order to determine whether heat shock proteins (HSP) can be used as indicators of environmental stress in the mammalian organism Peromyscus leucopus, three doses of each of two chemical stresses, cadmium chloride and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), were given intraperitoneally for 14 days. Proteins extracted from liver samples were compared by one-dimensional gel electrophoresis to liver protein samples from heat shocked and control animals. HSPs of 105,78, and 43 kDa were cadmium inducible. Non-heat shocked cadmium inducible proteins of 118, 55, and 47 kDa were seen. 2,4,5-T induced an increase in a 91 kDa protein. Low level cadmium induction of HSPs suggests useful applications in toxicological and general environmental tests.

Keywords

Environmental; Heat; Indicators; Proteins; Shock; Stress

Controlled Subject

Zoology; Molecular biology; Physiology

File Format

pdf

File Size

2017.28 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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