Award Date

1-1-1996

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

Number of Pages

79

Abstract

The focus of this project was to develop fluorescence-based immunoassays for the determination of 2,4-Dichlorophenoxyacetic acid (2,4-D). Three formats using polyclonal anti-2,4-D sera were developed, the first being a homogeneous, competitive assay based on the principle of fluorescence energy transfer. This assay exhibited a linear dynamic range of 100 ppb to 100 ppm; The next two formats are heterogeneous, competitive assays that were developed using a prototype KinExA{dollar}\rm\sp{TM}{dollar} immunoanalyzer. The assay formats use either antibody or antigen coated polymethylmethacrylate (PMMA) beads as a renewable solid phase. The KinExA{dollar}\rm\sp{TM}{dollar} assay was demonstrated in buffered saline solution as well as in aquatic environmental media, and exhibited a linear dynamic range of 2 ppb to 20 ppm. The antibodies showed a degree of cross-reactivity with compounds of closely related structure to 2,4-D, but little cross-reactivity with structurally dissimilar compounds. The KinExA{dollar}\rm\sp{TM}{dollar} methods are rapid (10 minutes per sample), simple, and potentially portable.

Keywords

Acid Determination; Development; Dichlorophenoxyacetic; Fluoroimmunoassays

Controlled Subject

Chemistry, Analytic; Biochemistry; Environmental sciences

File Format

pdf

File Size

1884.16 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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