Award Date
1-1-1989
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
First Committee Member
Stanley D. Hillyard
Number of Pages
90
Abstract
The amphibian antidiuretic hormone arginine vasotocin (AVT) stimulated osmotic water flow (JH{dollar}\sb2{dollar}O) across isolated skin from the pelvic but not the pectoral region of the toad, Bufo woodhousei. The apical membrane capacitance (C{dollar}\sb{\rm a}{dollar}) of the cells in the stratum granulosum of neither skin region was changed by AVT treatment when an osmotic gradient was present across the tissue; Short circuit current (I{dollar}\sb{\rm sc}{dollar}) and the amiloride sensitive current (I{dollar}\sb{\rm Na\sp+}{dollar}) both increased significantly in the pelvic and in the pectoral skin after AVT treatment, but to a larger degree in the pelvic skin. If M is expressed as a function of C{dollar}\sb{\rm a}{dollar}, the density of Na{dollar}\sp+{dollar} channels (assuming 1{dollar}\mu{dollar}F/cm{dollar}\sp2{dollar}) was 90 channels/{dollar}\mu{dollar}m{dollar}\sp2{dollar} of actual cell membrane area before AVT treatment and 273 channels/{dollar}\mu{dollar}m{dollar}\sp2{dollar} after AVT treatment. In the pectoral skin M did not increase significantly, however, I{dollar}\sb{\rm Na\sp+}{dollar} was linearly related to M. The current through single Na{dollar}\sp+{dollar} channels (i) did not change in either skin region after AVT treatment. (Abstract shortened with permission of author.).
Keywords
Bufo woodhousei; Capacitance; Channel; Comparison; Densities; Flux; Ion; Membrane Pectoral; Pelvic Skin; Sodium; Toad; Water; Woodhouse; AVT
Controlled Subject
Zoology; Biochemistry
File Format
File Size
2222.08 KB
Degree Grantor
University of Nevada, Las Vegas
Language
English
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Repository Citation
Baker, Cathleen Ann, "Comparison of the sodium ion channel densities, the water flux changes, and the membrane capacitance changes in the pelvic and pectoral skin of the toad, Bufo woodhousei" (1989). UNLV Retrospective Theses & Dissertations. 43.
http://dx.doi.org/10.25669/03ak-rwxz
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