Marinobacter strain NCE312 has a Pseudomonas-like naphthalene dioxygenase

Document Type

Article

Publication Date

7-2001

Publication Title

FEMS Microbiology Letters

Volume

201

Issue

1

First page number:

47

Last page number:

51

Abstract

One strain of bacteria, designated NCE312, was isolated from a naphthalene-digesting chemostat culture that was inoculated with creosote-contaminated marine sediment. The strain was isolated based on its ability to grow using naphthalene as a sole carbon source. In addition, the strain degraded 2-methylnaphthalene and 1-methylnaphthalene. Analysis of a 16S rRNA gene sequence from NCE312 placed the isolate in the genus Marinobacter. Degenerate PCR primers were used to amplify a fragment of a naphthalene 1,2-dioxygenase large subunit gene. A phylogenetic analysis indicated the Marinobacter naphthalene dioxygenase is similar to those from Pseudomonas and Burkholderia strains suggesting that the dioxygenase gene may have been transferred horizontally between these lineages of bacteria.

Keywords

Dioxygenase; Genetic transformation; Horizontal gene transfer; Marine bacteria; Marine bacterium; Marinobacter; Naphthalene; Oxygenases; Phylogeny; Polycyclic aromatic hydrocarbons; Polymerase chain reaction

Disciplines

Bacteriology | Ecology and Evolutionary Biology | Genetics and Genomics | Life Sciences | Microbiology

Language

English

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