Vibrio cyclotrophicus sp. nov., a polycyclic aromatic hydrocarbon (PAH)-degrading marine bacterium

Authors

Brian P. Hedlund, University of Nevada, Las VegasFollow
James T. Staley, University of Washington, SeattleFollow

Document Type

Article

Publication Date

1-2001

Publication Title

International Journal of Systematic and Evolutionary Microbiology

Volume

51

Issue

1

First page number:

61

Last page number:

66

Abstract

Strain P-2P44T was isolated from creosote-contaminated marine sediments by using a most-probable number procedure in which phenanthrene was the sole carbon and energy source. Growth experiments showed that P-2P44T utilized several two- and three-ring polycyclic aromatic hydrocarbons (PAHs) as substrates, including naphthalene, 2-methylnaphthalene and phenanthrene. Additionally, gas-chromatography experiments showed that P-2P44T degraded several other PAHs, though it was unable to use them as sole sources of carbon and energy. Phylogenetic analyses confirmed that strain P-2P44T is a member of the genus Vibrio, most closely related to Vibrio splendidus. However, strain P-2P44T shared only 98.3% 16S rDNA identity and 35% DNA-DNA reassociation with the type strain of V. splendidus. Strain P-2P44T differed phenotypically from V. splendidus. Together, these differences indicated that strain P-2P44T represents a novel species in the genus Vibrio, for which the name Vibrio cyclotrophicus sp. nov. is proposed; the type strain is P-2P44T (= ATCC 700982T = PICC 106644T).

Keywords

Marine bacteria; Phenanthrene; Phylogeny; Polycyclic aromatic hydrocarbons; Vibrio

Disciplines

Bacteriology | Ecology and Evolutionary Biology | Genetics and Genomics | Life Sciences | Marine Biology | Microbiology

Language

English

Repository Citation

Hedlund, B. P., Staley, J. T. (2001). Vibrio cyclotrophicus sp. nov., a polycyclic aromatic hydrocarbon (PAH)-degrading marine bacterium. International Journal of Systematic and Evolutionary Microbiology, 51(1), 61-66.
http://dx.doi.org/10.1099/00207713-51-1-61