Master of Science (MS)
First Committee Member
Second Committee Member
Third Committee Member
Fourth Committee Member
Number of Pages
Candidate bacterial phylum Omnitrophota has never been isolated and is poorly understood. In this study, we explored the genomics, phylogeny, taxonomy, distribution, cell size, and predicted physiology of Omnitrophota based on 316 genomes. Phylogenomic concordance identified seven classes accommodating 144 species. Calibration of genome-based taxonomy to ribosomal SSU profiling revealed most Omnitrophota classes are prevalent in the Earth Microbiome Dataset, particularly in soils and sediments. Fluorescence-activated cell sorting and differential size filtration experiments showed ultra-small (<400 >nm) cells are common across the phylum, as are parasitism-related genes, including a conserved tight adherence (Tad) complex and ATP/ADP translocase. However, nucleotide and amino acid biosynthetic pathways are not reduced, possibly suggesting early-stage or facultative intracellular parasitism. Energy metabolism is conserved within classes, with either acetogenic Wood-Ljungdahl or diverse respiratory pathways present. Large open reading frames (>20 kb) possibly involved in cell-cell adhesion are conserved across the phylum, including the largest prokaryotic ORF predicted yet (140 kb). These results demonstrate Omnitrophota are diverse and ubiquitous, including putative syntrophs, predators, and free-living nanobacteria that encode unusual giant open-reading frames.
Microbial ecology; Omnitrophota
University of Nevada, Las Vegas
Seymour, Cale O., "Analysis of 316 Omnitrophota (OP3) Genomes Reveals a Diverse and Ubiquitous Candidate Phylum of Putative Syntrophs, Predators, and Free-Living Nanobacteria Encoding Giant Open-Reading Frames." (2021). UNLV Theses, Dissertations, Professional Papers, and Capstones. 4263.
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