Award Date

5-1-2019

Degree Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Kinesiology and Nutrition Sciences

First Committee Member

James . Navalta

Second Committee Member

Brian . Schilling

Third Committee Member

Jennifer . Kawi

Fourth Committee Member

Timothy . Bungum

Number of Pages

76

Abstract

Introduction: Investigations of lipid metabolism commonly utilize an oral fat tolerance test (OFTT) to elicit a dramatic increase in serum triglycerides (TG). Although this is common practice there does not currently exist an agreed upon formula for an OFTT. Nor has there been in investigation as to the reliability among commonly used OFTT. Furthermore, the effects of sex and activity level are mixed among the literature. The purpose of this investigation is to discover an ideal OFTT for research and clinical purposes, evaluate the effects of activity and sex on TG metabolism, and to test each OFTT concentration for reliability.

Methods: A sample of 30 healthy college-aged adults consumed three different OFTT, with a seven-day washout. The population consisted of 8 active males, 9 active females, 7 sedentary males, and 6 sedentary females. The OFTT consisted of fat concentrations approximating 50g, 100g, and 150g. An additional OFTT of one of the aforementioned concentrations was administered to determine reliability.

Results: Plasma TG was lower among the 50g load compared (1h = 134±20, 2h = 140±15, 3h = 130±15, 4h = 125±20 mg/dL) compared to the 100g (1h = 170±20, 2h = 180±25, 3h = 170±25, 4h = 160±20 mg/dL) and 150g loads (1h = 175±20, 2h = 200±25, 3h = 180±30, 4h = 175±20 mg/dL) (F=1.508, p=0.033). Females showed greater attenuation of TG among than did males within the 100g load from the 2-3-hour time point Females 3h = 152±71, Males 3h = 186±106)(F=3.115, p=0.049). The 100g load has the greatest reliability outcome, with an ICC of 0.924 (p<0.001).

Conclusion: The proposed 100g OFTT load is ideal for lipid testing and has shown to be valid and reliable. Persons maintaining an active lifestyle metabolize TG more efficiently than their sedentary counterparts. No difference of TG metabolism is present between sex.

Keywords

Lipid metabolism; Metabolism; OFTT; Oral fat tolerance test; Reliability

Disciplines

Kinesiology

File Format

pdf

Degree Grantor

University of Nevada, Las Vegas

Language

English

Rights

IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

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